H2N2V1, 1957, Analysis, bibRecord, 000032

Swine Influenza Virus PA and Neuraminidase Gene Reassortment into Human H1N1 Influenza Virus Is Associated with an Altered Pathogenic Phenotype Linked to Increased MIP-2 Expression

Identifieur interne : 000032 ( 1957/Analysis ); précédent : 000031; suivant : 000033

Swine Influenza Virus PA and Neuraminidase Gene Reassortment into Human H1N1 Influenza Virus Is Associated with an Altered Pathogenic Phenotype Linked to Increased MIP-2 Expression

Auteurs : Daniel Dlugolenski [États-Unis] ; Les Jones [États-Unis] ; Elizabeth Howerth [États-Unis] ; David Wentworth [États-Unis] ; S. Mark Tompkins [États-Unis] ; Ralph A. Tripp [États-Unis]

Source :

Journal of Virology [ 0022-538X ] ; 2015.

RBID : PMC:4442520

Descripteurs français

English descriptors

KwdEn :
- Amino Acid Sequence, Animals, Cell Line, Chemokine CXCL2 (genetics), Cytokines (biosynthesis), Female, Ferrets, Hemagglutinin Glycoproteins, Influenza Virus (genetics), Host Specificity (genetics), Humans, Immunity, Innate, Influenza A Virus, H1N1 Subtype (genetics), Influenza A Virus, H1N1 Subtype (pathogenicity), Influenza A Virus, H1N2 Subtype (genetics), Influenza A Virus, H1N2 Subtype (pathogenicity), Influenza, Human (virology), Killer Cells, Natural (immunology), Lung (immunology), Lung (pathology), Lymphocyte Activation, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Neuraminidase (genetics), Neutrophil Infiltration, Orthomyxoviridae Infections (immunology), Orthomyxoviridae Infections (veterinary), Orthomyxoviridae Infections (virology), Phenotype, RNA Replicase (genetics), Reassortant Viruses (genetics), Reassortant Viruses (pathogenicity), Sequence Homology, Amino Acid, Swine (virology), Swine Diseases (virology), T-Lymphocytes (immunology), Up-Regulation, Viral Proteins (genetics), Virulence (genetics).
MESH :
- chemical , biosynthesis : Cytokines.
- chemical , genetics : Chemokine CXCL2, Hemagglutinin Glycoproteins, Influenza Virus, Neuraminidase, RNA Replicase, Viral Proteins.
- genetics : Host Specificity, Influenza A Virus, H1N1 Subtype, Influenza A Virus, H1N2 Subtype, Reassortant Viruses, Virulence.
- immunology : Killer Cells, Natural, Lung, Orthomyxoviridae Infections, T-Lymphocytes.
- pathogenicity : Influenza A Virus, H1N1 Subtype, Influenza A Virus, H1N2 Subtype, Reassortant Viruses.
- pathology : Lung.
- veterinary : Orthomyxoviridae Infections.
- virology : Influenza, Human, Orthomyxoviridae Infections, Swine, Swine Diseases.
- Amino Acid Sequence, Animals, Cell Line, Female, Ferrets, Humans, Immunity, Innate, Lymphocyte Activation, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Neutrophil Infiltration, Phenotype, Sequence Homology, Amino Acid, Up-Regulation.

Abstract

ABSTRACT

Swine are susceptible to infection by both avian and human influenza viruses, and this feature is thought to contribute to novel reassortant influenza viruses. In this study, the influenza virus reassortment rate in swine and human cells was determined. Coinfection of swine cells with 2009 pandemic H1N1 virus (huH1N1) and an endemic swine H1N2 (A/swine/Illinois/02860/09) virus (swH1N2) resulted in a 23% reassortment rate that was independent of α2,3- or α2,6-sialic acid distribution on the cells. The reassortants had altered pathogenic phenotypes linked to introduction of the swine virus PA and neuraminidase (NA) into huH1N1. In mice, the huH1N1 PA and NA mediated increased MIP-2 expression early postinfection, resulting in substantial pulmonary neutrophilia with enhanced lung pathology and disease. The findings support the notion that swine are a mixing vessel for influenza virus reassortants independent of sialic acid distribution. These results show the potential for continued reassortment of the 2009 pandemic H1N1 virus with endemic swine viruses and for reassortants to have increased pathogenicity linked to the swine virus NA and PA genes which are associated with increased pulmonary neutrophil trafficking that is related to MIP-2 expression.

IMPORTANCE Influenza A viruses can change rapidly via reassortment to create a novel virus, and reassortment can result in possible pandemics. Reassortments among subtypes from avian and human viruses led to the 1957 (H2N2 subtype) and 1968 (H3N2 subtype) human influenza pandemics. Recent analyses of circulating isolates have shown that multiple genes can be recombined from human, avian, and swine influenza viruses, leading to triple reassortants. Understanding the factors that can affect influenza A virus reassortment is needed for the establishment of disease intervention strategies that may reduce or preclude pandemics. The findings from this study show that swine cells provide a mixing vessel for influenza virus reassortment independent of differential sialic acid distribution. The findings also establish that circulating neuraminidase (NA) and PA genes could alter the pathogenic phenotype of the pandemic H1N1 virus, resulting in enhanced disease. The identification of such factors provides a framework for pandemic modeling and surveillance.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4442520

DOI: 10.1128/JVI.00087-15
PubMed: 25762737
PubMed Central: 4442520

Affiliations:

Links to Exploration step

PMC:4442520

Le document en format XML

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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Swine Influenza Virus PA and Neuraminidase Gene Reassortment into Human H1N1 Influenza Virus Is Associated with an Altered Pathogenic Phenotype Linked to Increased MIP-2 Expression</title>
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<term>Animals</term>
<term>Cell Line</term>
<term>Chemokine CXCL2 (genetics)</term>
<term>Cytokines (biosynthesis)</term>
<term>Female</term>
<term>Ferrets</term>
<term>Hemagglutinin Glycoproteins, Influenza Virus (genetics)</term>
<term>Host Specificity (genetics)</term>
<term>Humans</term>
<term>Immunity, Innate</term>
<term>Influenza A Virus, H1N1 Subtype (genetics)</term>
<term>Influenza A Virus, H1N1 Subtype (pathogenicity)</term>
<term>Influenza A Virus, H1N2 Subtype (genetics)</term>
<term>Influenza A Virus, H1N2 Subtype (pathogenicity)</term>
<term>Influenza, Human (virology)</term>
<term>Killer Cells, Natural (immunology)</term>
<term>Lung (immunology)</term>
<term>Lung (pathology)</term>
<term>Lymphocyte Activation</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Molecular Sequence Data</term>
<term>Neuraminidase (genetics)</term>
<term>Neutrophil Infiltration</term>
<term>Orthomyxoviridae Infections (immunology)</term>
<term>Orthomyxoviridae Infections (veterinary)</term>
<term>Orthomyxoviridae Infections (virology)</term>
<term>Phenotype</term>
<term>RNA Replicase (genetics)</term>
<term>Reassortant Viruses (genetics)</term>
<term>Reassortant Viruses (pathogenicity)</term>
<term>Sequence Homology, Amino Acid</term>
<term>Swine (virology)</term>
<term>Swine Diseases (virology)</term>
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<term>Cellules tueuses naturelles (immunologie)</term>
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<term>Cytokines (biosynthèse)</term>
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<term>Furets</term>
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<term>Infections à Orthomyxoviridae (immunologie)</term>
<term>Infections à Orthomyxoviridae (médecine vétérinaire)</term>
<term>Infections à Orthomyxoviridae (virologie)</term>
<term>Infiltration par les neutrophiles</term>
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<term>Poumon (immunologie)</term>
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<term>Régulation positive</term>
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<term>Similitude de séquences d'acides aminés</term>
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<term>Souris de lignée BALB C</term>
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<term>Séquence d'acides aminés</term>
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<term>RNA Replicase</term>
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<term>Influenza A Virus, H1N2 Subtype</term>
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<term>Virulence</term>
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<term>RNA replicase</term>
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<term>Spécificité d'hôte</term>
<term>Virulence</term>
<term>Virus recombinants</term>
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<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Cellules tueuses naturelles</term>
<term>Infections à Orthomyxoviridae</term>
<term>Lymphocytes T</term>
<term>Poumon</term>
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<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Killer Cells, Natural</term>
<term>Lung</term>
<term>Orthomyxoviridae Infections</term>
<term>T-Lymphocytes</term>
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<term>Sous-type H1N2 du virus de la grippe A</term>
<term>Virus recombinants</term>
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<term>Maladies des porcs</term>
<term>Suidae</term>
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<term>Swine</term>
<term>Swine Diseases</term>
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<term>Animals</term>
<term>Cell Line</term>
<term>Female</term>
<term>Ferrets</term>
<term>Humans</term>
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<term>Lymphocyte Activation</term>
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<term>Up-Regulation</term>
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<term>Données de séquences moléculaires</term>
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<term>Furets</term>
<term>Humains</term>
<term>Immunité innée</term>
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<term>Similitude de séquences d'acides aminés</term>
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<front><div type="abstract" xml:lang="en"><title>ABSTRACT</title>
<p>Swine are susceptible to infection by both avian and human influenza viruses, and this feature is thought to contribute to novel reassortant influenza viruses. In this study, the influenza virus reassortment rate in swine and human cells was determined. Coinfection of swine cells with 2009 pandemic H1N1 virus (huH1N1) and an endemic swine H1N2 (A/swine/Illinois/02860/09) virus (swH1N2) resulted in a 23% reassortment rate that was independent of α2,3- or α2,6-sialic acid distribution on the cells. The reassortants had altered pathogenic phenotypes linked to introduction of the swine virus PA and neuraminidase (NA) into huH1N1. In mice, the huH1N1 PA and NA mediated increased MIP-2 expression early postinfection, resulting in substantial pulmonary neutrophilia with enhanced lung pathology and disease. The findings support the notion that swine are a mixing vessel for influenza virus reassortants independent of sialic acid distribution. These results show the potential for continued reassortment of the 2009 pandemic H1N1 virus with endemic swine viruses and for reassortants to have increased pathogenicity linked to the swine virus NA and PA genes which are associated with increased pulmonary neutrophil trafficking that is related to MIP-2 expression.</p>
<p><bold>IMPORTANCE</bold>
 Influenza A viruses can change rapidly via reassortment to create a novel virus, and reassortment can result in possible pandemics. Reassortments among subtypes from avian and human viruses led to the 1957 (H2N2 subtype) and 1968 (H3N2 subtype) human influenza pandemics. Recent analyses of circulating isolates have shown that multiple genes can be recombined from human, avian, and swine influenza viruses, leading to triple reassortants. Understanding the factors that can affect influenza A virus reassortment is needed for the establishment of disease intervention strategies that may reduce or preclude pandemics. The findings from this study show that swine cells provide a mixing vessel for influenza virus reassortment independent of differential sialic acid distribution. The findings also establish that circulating neuraminidase (NA) and PA genes could alter the pathogenic phenotype of the pandemic H1N1 virus, resulting in enhanced disease. The identification of such factors provides a framework for pandemic modeling and surveillance.</p>
</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Géorgie (États-Unis)</li>
<li>Maryland</li>
</region>
</list>
<tree><country name="États-Unis"><region name="Géorgie (États-Unis)"><name sortKey="Dlugolenski, Daniel" sort="Dlugolenski, Daniel" uniqKey="Dlugolenski D" first="Daniel" last="Dlugolenski">Daniel Dlugolenski</name>
</region>
<name sortKey="Howerth, Elizabeth" sort="Howerth, Elizabeth" uniqKey="Howerth E" first="Elizabeth" last="Howerth">Elizabeth Howerth</name>
<name sortKey="Jones, Les" sort="Jones, Les" uniqKey="Jones L" first="Les" last="Jones">Les Jones</name>
<name sortKey="Tompkins, S Mark" sort="Tompkins, S Mark" uniqKey="Tompkins S" first="S. Mark" last="Tompkins">S. Mark Tompkins</name>
<name sortKey="Tripp, Ralph A" sort="Tripp, Ralph A" uniqKey="Tripp R" first="Ralph A." last="Tripp">Ralph A. Tripp</name>
<name sortKey="Wentworth, David" sort="Wentworth, David" uniqKey="Wentworth D" first="David" last="Wentworth">David Wentworth</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/1957/Analysis

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000032 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/1957/Analysis/biblio.hfd -nk 000032 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    H2N2V1
   |flux=    1957
   |étape=   Analysis
   |type=    RBID
   |clé=     PMC:4442520
   |texte=   Swine Influenza Virus PA and Neuraminidase Gene Reassortment into Human H1N1 Influenza Virus Is Associated with an Altered Pathogenic Phenotype Linked to Increased MIP-2 Expression
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/1957/Analysis/RBID.i   -Sk "pubmed:25762737" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/1957/Analysis/biblio.hfd   \
       | NlmPubMed2Wicri -a H2N2V1

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 14 19:59:40 2020. Site generation: Thu Mar 25 15:38:26 2021

Serveur d'exploration H2N2

Swine Influenza Virus PA and Neuraminidase Gene Reassortment into Human H1N1 Influenza Virus Is Associated with an Altered Pathogenic Phenotype Linked to Increased MIP-2 Expression

Swine Influenza Virus PA and Neuraminidase Gene Reassortment into Human H1N1 Influenza Virus Is Associated with an Altered Pathogenic Phenotype Linked to Increased MIP-2 Expression

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Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration H2N2
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